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EuroClone cycle-pure kit
Cycle Pure Kit, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycle-pure+kit/cycle+pure+kit/bio_rxiv__2025__07__17__664916-72-9-12
Average 90 stars, based on 1 article reviews
cycle-pure kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Phylogeny of Neobursaridium reshapes the systematics of Paramecium (Oligohymenophorea, Ciliophora)
Article Snippet: 1Department of Biology, University of Pisa, Pisa, Italy 2Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia 3St.. Petersburg Branch of the S.I.. Vavilov Institute of History of Science and Technology Russian Academy of Sciences, St. Petersburg, Russia 4Department of Biology and Biotechnology ‘Lazzaro Spallanzani’, Pavia University, Pavia, Italy 5National Centre for Coastal Research, Ministry of Earth Sciences, Government of India, NIOT Campus, Pallikaranai, Chennai, India 6Department of Zoology, Andhra University, Visakhapatnam, India 7Department of Biotechnology, Andhra University, Visakhapatnam, India 8CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy 9CISUP, Centro per l'Integrazione della Strumentazione dell'Università di Pisa, Pisa, Italy

Article Title: Description of three new predator species of Litostomatea (Alveolata, Ciliophora), including sequencing and annotation of their mitochondrial genomes
Article Snippet: The so obtained PCR products were purified with the Eurogold Cycle-Pure Kit (EuroClone, Milan, Italy) and subsequently sent for direct sequencing to an external sequencing company (GENEWIZ Germany GmbH (Leipzig, Germany), by adding appropriate internal primers: 18S R536 (5’-CTG GAA TTA CCG CGG CTG-3’), 18S R1052 (5’-AAC TAA GAA CGG CCA TGC A-3’), 18S F783 (5’-GAC GAT CAG ATA CCG TC-3’) ( ).

Article Title: Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.
Article Snippet: Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time.. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time.. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues.

Article Title: Single-cell Microbiomics Unveils Distribution and Patterns of Microbial Symbioses in the Natural Environment.
Article Snippet: Protist-bacteria associations are extremely common.. Among them, those involving ciliates of the genus Euplotes are emerging as models for symbioses between prokaryotes and eukaryotes, and a great deal of information is available from cultured representatives of this system.. Even so, as for most known microbial symbioses, data on natural populations is lacking, and their ecology remains largely unexplored; how well lab cultures represent actual diversity is untested.

Article Title: Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.
Article Snippet: All PCR products from canary DNA were purified by EuroGOLD Cycle-Pure Kit (EuroClone, Milan, Italy).

Article Title: The Hidden World of Rickettsiales Symbionts: "Candidatus Spectririckettsia obscura," a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum.
Article Snippet: Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners.. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas.. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas.

Purification:

Article Title: Phylogeny of Neobursaridium reshapes the systematics of Paramecium (Oligohymenophorea, Ciliophora)
Article Snippet: 1Department of Biology, University of Pisa, Pisa, Italy 2Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia 3St.. Petersburg Branch of the S.I.. Vavilov Institute of History of Science and Technology Russian Academy of Sciences, St. Petersburg, Russia 4Department of Biology and Biotechnology ‘Lazzaro Spallanzani’, Pavia University, Pavia, Italy 5National Centre for Coastal Research, Ministry of Earth Sciences, Government of India, NIOT Campus, Pallikaranai, Chennai, India 6Department of Zoology, Andhra University, Visakhapatnam, India 7Department of Biotechnology, Andhra University, Visakhapatnam, India 8CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy 9CISUP, Centro per l'Integrazione della Strumentazione dell'Università di Pisa, Pisa, Italy

Article Title: Description of three new predator species of Litostomatea (Alveolata, Ciliophora), including sequencing and annotation of their mitochondrial genomes
Article Snippet: The so obtained PCR products were purified with the Eurogold Cycle-Pure Kit (EuroClone, Milan, Italy) and subsequently sent for direct sequencing to an external sequencing company (GENEWIZ Germany GmbH (Leipzig, Germany), by adding appropriate internal primers: 18S R536 (5’-CTG GAA TTA CCG CGG CTG-3’), 18S R1052 (5’-AAC TAA GAA CGG CCA TGC A-3’), 18S F783 (5’-GAC GAT CAG ATA CCG TC-3’) ( ).

Article Title: Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.
Article Snippet: Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time.. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time.. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues.

Article Title: Single-cell Microbiomics Unveils Distribution and Patterns of Microbial Symbioses in the Natural Environment.
Article Snippet: Protist-bacteria associations are extremely common.. Among them, those involving ciliates of the genus Euplotes are emerging as models for symbioses between prokaryotes and eukaryotes, and a great deal of information is available from cultured representatives of this system.. Even so, as for most known microbial symbioses, data on natural populations is lacking, and their ecology remains largely unexplored; how well lab cultures represent actual diversity is untested.

Article Title: Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.
Article Snippet: All PCR products from canary DNA were purified by EuroGOLD Cycle-Pure Kit (EuroClone, Milan, Italy).

Article Title: The Hidden World of Rickettsiales Symbionts: "Candidatus Spectririckettsia obscura," a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum.
Article Snippet: Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners.. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas.. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas.

Sequencing:

Article Title: Phylogeny of Neobursaridium reshapes the systematics of Paramecium (Oligohymenophorea, Ciliophora)
Article Snippet: 1Department of Biology, University of Pisa, Pisa, Italy 2Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia 3St.. Petersburg Branch of the S.I.. Vavilov Institute of History of Science and Technology Russian Academy of Sciences, St. Petersburg, Russia 4Department of Biology and Biotechnology ‘Lazzaro Spallanzani’, Pavia University, Pavia, Italy 5National Centre for Coastal Research, Ministry of Earth Sciences, Government of India, NIOT Campus, Pallikaranai, Chennai, India 6Department of Zoology, Andhra University, Visakhapatnam, India 7Department of Biotechnology, Andhra University, Visakhapatnam, India 8CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy 9CISUP, Centro per l'Integrazione della Strumentazione dell'Università di Pisa, Pisa, Italy

Article Title: Description of three new predator species of Litostomatea (Alveolata, Ciliophora), including sequencing and annotation of their mitochondrial genomes
Article Snippet: The so obtained PCR products were purified with the Eurogold Cycle-Pure Kit (EuroClone, Milan, Italy) and subsequently sent for direct sequencing to an external sequencing company (GENEWIZ Germany GmbH (Leipzig, Germany), by adding appropriate internal primers: 18S R536 (5’-CTG GAA TTA CCG CGG CTG-3’), 18S R1052 (5’-AAC TAA GAA CGG CCA TGC A-3’), 18S F783 (5’-GAC GAT CAG ATA CCG TC-3’) ( ).

Article Title: Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.
Article Snippet: Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time.. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time.. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues.

Article Title: Single-cell Microbiomics Unveils Distribution and Patterns of Microbial Symbioses in the Natural Environment.
Article Snippet: Protist-bacteria associations are extremely common.. Among them, those involving ciliates of the genus Euplotes are emerging as models for symbioses between prokaryotes and eukaryotes, and a great deal of information is available from cultured representatives of this system.. Even so, as for most known microbial symbioses, data on natural populations is lacking, and their ecology remains largely unexplored; how well lab cultures represent actual diversity is untested.

Article Title: Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.
Article Snippet: All PCR products from canary DNA were purified by EuroGOLD Cycle-Pure Kit (EuroClone, Milan, Italy).

Article Title: The Hidden World of Rickettsiales Symbionts: "Candidatus Spectririckettsia obscura," a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum.
Article Snippet: Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners.. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas.. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas.

Bacteria:

Article Title: Phylogeny of Neobursaridium reshapes the systematics of Paramecium (Oligohymenophorea, Ciliophora)
Article Snippet: 1Department of Biology, University of Pisa, Pisa, Italy 2Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia 3St.. Petersburg Branch of the S.I.. Vavilov Institute of History of Science and Technology Russian Academy of Sciences, St. Petersburg, Russia 4Department of Biology and Biotechnology ‘Lazzaro Spallanzani’, Pavia University, Pavia, Italy 5National Centre for Coastal Research, Ministry of Earth Sciences, Government of India, NIOT Campus, Pallikaranai, Chennai, India 6Department of Zoology, Andhra University, Visakhapatnam, India 7Department of Biotechnology, Andhra University, Visakhapatnam, India 8CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy 9CISUP, Centro per l'Integrazione della Strumentazione dell'Università di Pisa, Pisa, Italy

Article Title: Description of three new predator species of Litostomatea (Alveolata, Ciliophora), including sequencing and annotation of their mitochondrial genomes
Article Snippet: The so obtained PCR products were purified with the Eurogold Cycle-Pure Kit (EuroClone, Milan, Italy) and subsequently sent for direct sequencing to an external sequencing company (GENEWIZ Germany GmbH (Leipzig, Germany), by adding appropriate internal primers: 18S R536 (5’-CTG GAA TTA CCG CGG CTG-3’), 18S R1052 (5’-AAC TAA GAA CGG CCA TGC A-3’), 18S F783 (5’-GAC GAT CAG ATA CCG TC-3’) ( ).

Article Title: Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.
Article Snippet: Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time.. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time.. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues.

Article Title: Single-cell Microbiomics Unveils Distribution and Patterns of Microbial Symbioses in the Natural Environment.
Article Snippet: Protist-bacteria associations are extremely common.. Among them, those involving ciliates of the genus Euplotes are emerging as models for symbioses between prokaryotes and eukaryotes, and a great deal of information is available from cultured representatives of this system.. Even so, as for most known microbial symbioses, data on natural populations is lacking, and their ecology remains largely unexplored; how well lab cultures represent actual diversity is untested.

Article Title: Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.
Article Snippet: All PCR products from canary DNA were purified by EuroGOLD Cycle-Pure Kit (EuroClone, Milan, Italy).

Article Title: The Hidden World of Rickettsiales Symbionts: "Candidatus Spectririckettsia obscura," a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum.
Article Snippet: Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners.. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas.. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas.

Clone Assay:

Article Title: Phylogeny of Neobursaridium reshapes the systematics of Paramecium (Oligohymenophorea, Ciliophora)
Article Snippet: 1Department of Biology, University of Pisa, Pisa, Italy 2Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia 3St.. Petersburg Branch of the S.I.. Vavilov Institute of History of Science and Technology Russian Academy of Sciences, St. Petersburg, Russia 4Department of Biology and Biotechnology ‘Lazzaro Spallanzani’, Pavia University, Pavia, Italy 5National Centre for Coastal Research, Ministry of Earth Sciences, Government of India, NIOT Campus, Pallikaranai, Chennai, India 6Department of Zoology, Andhra University, Visakhapatnam, India 7Department of Biotechnology, Andhra University, Visakhapatnam, India 8CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy 9CISUP, Centro per l'Integrazione della Strumentazione dell'Università di Pisa, Pisa, Italy

Article Title: Description of three new predator species of Litostomatea (Alveolata, Ciliophora), including sequencing and annotation of their mitochondrial genomes
Article Snippet: The so obtained PCR products were purified with the Eurogold Cycle-Pure Kit (EuroClone, Milan, Italy) and subsequently sent for direct sequencing to an external sequencing company (GENEWIZ Germany GmbH (Leipzig, Germany), by adding appropriate internal primers: 18S R536 (5’-CTG GAA TTA CCG CGG CTG-3’), 18S R1052 (5’-AAC TAA GAA CGG CCA TGC A-3’), 18S F783 (5’-GAC GAT CAG ATA CCG TC-3’) ( ).

Article Title: Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.
Article Snippet: Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time.. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time.. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues.

Article Title: Single-cell Microbiomics Unveils Distribution and Patterns of Microbial Symbioses in the Natural Environment.
Article Snippet: Protist-bacteria associations are extremely common.. Among them, those involving ciliates of the genus Euplotes are emerging as models for symbioses between prokaryotes and eukaryotes, and a great deal of information is available from cultured representatives of this system.. Even so, as for most known microbial symbioses, data on natural populations is lacking, and their ecology remains largely unexplored; how well lab cultures represent actual diversity is untested.

Article Title: Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.
Article Snippet: All PCR products from canary DNA were purified by EuroGOLD Cycle-Pure Kit (EuroClone, Milan, Italy).

Article Title: The Hidden World of Rickettsiales Symbionts: "Candidatus Spectririckettsia obscura," a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum.
Article Snippet: Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners.. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas.. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas.

Ligation:

Article Title: Phylogeny of Neobursaridium reshapes the systematics of Paramecium (Oligohymenophorea, Ciliophora)
Article Snippet: 1Department of Biology, University of Pisa, Pisa, Italy 2Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia 3St.. Petersburg Branch of the S.I.. Vavilov Institute of History of Science and Technology Russian Academy of Sciences, St. Petersburg, Russia 4Department of Biology and Biotechnology ‘Lazzaro Spallanzani’, Pavia University, Pavia, Italy 5National Centre for Coastal Research, Ministry of Earth Sciences, Government of India, NIOT Campus, Pallikaranai, Chennai, India 6Department of Zoology, Andhra University, Visakhapatnam, India 7Department of Biotechnology, Andhra University, Visakhapatnam, India 8CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy 9CISUP, Centro per l'Integrazione della Strumentazione dell'Università di Pisa, Pisa, Italy

Article Title: Description of three new predator species of Litostomatea (Alveolata, Ciliophora), including sequencing and annotation of their mitochondrial genomes
Article Snippet: The so obtained PCR products were purified with the Eurogold Cycle-Pure Kit (EuroClone, Milan, Italy) and subsequently sent for direct sequencing to an external sequencing company (GENEWIZ Germany GmbH (Leipzig, Germany), by adding appropriate internal primers: 18S R536 (5’-CTG GAA TTA CCG CGG CTG-3’), 18S R1052 (5’-AAC TAA GAA CGG CCA TGC A-3’), 18S F783 (5’-GAC GAT CAG ATA CCG TC-3’) ( ).

Article Title: Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.
Article Snippet: Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time.. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time.. Here, we present a pilot study using a single-cell microbiomic approach to circumvent these issues.

Article Title: Single-cell Microbiomics Unveils Distribution and Patterns of Microbial Symbioses in the Natural Environment.
Article Snippet: Protist-bacteria associations are extremely common.. Among them, those involving ciliates of the genus Euplotes are emerging as models for symbioses between prokaryotes and eukaryotes, and a great deal of information is available from cultured representatives of this system.. Even so, as for most known microbial symbioses, data on natural populations is lacking, and their ecology remains largely unexplored; how well lab cultures represent actual diversity is untested.

Article Title: Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.
Article Snippet: All PCR products from canary DNA were purified by EuroGOLD Cycle-Pure Kit (EuroClone, Milan, Italy).

Article Title: The Hidden World of Rickettsiales Symbionts: "Candidatus Spectririckettsia obscura," a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum.
Article Snippet: Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners.. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas.. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas.



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